How can I get this science article?

[IR5FORMUMSIE 850]

Look for GroEL (Hsp60) + cell distribution. I think that physiologically speaking, whatever Hsp60 could be doing cytosolically can be upregulated using other pathways that might not need so much manipulation from a clinical perspective.

I will, but you know that I won't be sleeping tonight thinking about this. In my mind I keep imagining some brute force form of Le Chatelier's principle and just pumping so many healthy hsp60 units in there that there might be some turnover and the active units would somehow replace the defective units. Just one of the many things that I thought of.

[HAL 9000 96]

Look for GroEL (Hsp60) + cell distribution. I think that physiologically speaking, whatever Hsp60 could be doing cytosolically can be upregulated using other pathways that might not need so much manipulation from a clinical perspective.

I will, but you know that I won't be sleeping tonight thinking about this. In my mind I keep imagining some brute force form of Le Chatelier's principle and just pumping so many healthy hsp60 units in there that there might be some turnover and the active units would somehow replace the defective units. Just one of the many things that I thought of.

Just admit it. What you want is for me to ship... lets say... some attenuated Adenovirus strain with... say... a CMV-driven Hsp60 cassette... for you to inject into people. Sick I tell you!!!

[Niels Bohr 72]

Maybe one day I get to work in that field. Converting protein into something of use with computers. In real life, if you meet an Asian (who is a Cambodian) guy at any physiology conference who is from Massachusetts, mention VJ. I like to work with cellular structures someday. Not with medicinal (since I'm not an expert) purposes, but for computer research purposes.

Edited November 21, 2009 by Niels Bohr

[HAL 9000 96]

Ok my friend.

[Niels Bohr 72]

Ok my friend.

[IR5FORMUMSIE 850]

Look for GroEL (Hsp60) + cell distribution. I think that physiologically speaking, whatever Hsp60 could be doing cytosolically can be upregulated using other pathways that might not need so much manipulation from a clinical perspective.

I will, but you know that I won't be sleeping tonight thinking about this. In my mind I keep imagining some brute force form of Le Chatelier's principle and just pumping so many healthy hsp60 units in there that there might be some turnover and the active units would somehow replace the defective units. Just one of the many things that I thought of.

Just admit it. What you want is for me to ship... lets say... some attenuated Adenovirus strain with... say... a CMV-driven Hsp60 cassette... for you to inject into people. Sick I tell you!!!

Was I that obvious? I mean perish the thought I would never do such a thing. Psst, how soon can you ship?

Maybe one day I get to work in that field. Converting protein into something of use with computers. In real life, if you meet an Asian (who is a Cambodian) guy at any physiology conference who is from Massachusetts, mention VJ. I like to work with cellular structures someday. Not with medicinal (since I'm not an expert) purposes, but for computer research purposes.

How many times have I heard that excuse.

[Niels Bohr 72]

[malego77 2]

Hi guys. I see you've been having a very good scientists discussion over here!

I am going to tell you the same thing "I am not an expert" in this topic... so I can't contribute to science now ha ha!

Thank you all for helping me, it should be great if you can request the article for me. I agree, it's a shame that only certain "hosters" (I don't know if it's the right name) or databases carry this kind of articles...

man, why getting into science has to be hard? science has to be for free!

[IR5FORMUMSIE 850]

Hi guys. I see you've been having a very good scientists discussion over here!

I am going to tell you the same thing "I am not an expert" in this topic... so I can't contribute to science now ha ha!

Thank you all for helping me, it should be great if you can request the article for me. I agree, it's a shame that only certain "hosters" (I don't know if it's the right name) or databases carry this kind of articles...

man, why getting into science has to be hard? science has to be for free!

You could lend us your brain, for a few minutes, we'll return it in good shape i promise.

Science should be free.

Here's the abstract and summary, I've embolded the text that has the contact info for the researchers.

Int J Hyperthermia. 2009 Jun;25(4):280-8.

Whole body hyperthermia reduces oxidative stress in the striatum of rats in an animal model of mitochondrial toxicity with 3-nitropropionic acid.

Medina-Navarro R, Guerrero-Linares I.

Department of Experimental Metabolism, Center for Biomedical Research of Michoacán (CIBIMI-IMSS), C.P. 58261, Morelia, Michoacán, México. rafael.medina@imss.gob.mx

PURPOSE: The goal of this study was to determine whether whole body hyperthermia (WBH) could reduce oxidative stress in the striatum produced by 3-nitropropionic acid (3-NP), a mitochondrial toxin that irreversibly inhibits succinate dehydrogenase (SDH), causing impairment of energy metabolism, oxidative stress and a selective degeneration of striatal cells. METHODS: Rats were subjected to WBH (42 degrees C) or normothermia control conditions for 30 min and then treated with 3-NP. Striatum samples were processed and the levels of protein carbonyl groups, biogenic amines, Hsp72 and salicylate hydroxylation (to probe the hydroxyl radical (OH(*)) intervention) were determined. RESULTS: WBH significantly reduced oxidative stress in the striatum of animals treated with 3-NP, as judged by reductions in protein carbonyl and salicylate hydroxylation derivative levels, whereas striatal Hsp72 expression was significantly increased. The groups treated with 3-NP presented an increased in the dopamine (DA) derivatives 2,3-dihydroxyphenylacetic acid (DOPAC) and norepinephrine (NE) concentration, whereas the striatal relation DOPAC/DA concentration indicate a reduced dopamine turnover. CONCLUSIONS: These studies show, for the first time, that a heat shock pretreatment can ameliorate the oxidative stress produced by a metabolic toxin (3-NP) capable of impairing energy supply and produce selective striatal degeneration. These data contribute to a better understanding of the potential for thermal stress to modulate the type of oxidative stress usually present in neurodegenerative disorders associated with metabolic defects.

Summary

International Journal of Hyperthermia

2009, Vol. 25, No. 4, Pages 280-288

Whole body hyperthermia reduces oxidative stress in the striatum of rats in an animal model of mitochondrial toxicity with 3-nitropropionic acid

DrRafael Medina-Navarro‌1† and Israel Guerrero-Linares‌1,2

1Department of Experimental Metabolism, Center for Biomedical Research of Michoacán (CIBIMI-IMSS), Michoacán, México

2Graduate Program in Pharmacology, University of Michoacán (Universidad Michoacana de San Nicolás de Hidalgo, UMSNH), Michoacán, México

†Correspondence: DrRafael Medina-Navarro, Centro de Investigación Biomédica de Michoacán, CIBIMI, Universidad Michoacana de San Nicolás de Hidalgo, Calzada Ventura Puente esq. Rafael Carrillo, PO Box 7-70, C.P. 58261, Morelia, Mich, México, (52) (443) 3241610 rafael.medina@imss.gob.mx

Purpose: The goal of this study was to determine whether whole body hyperthermia (WBH) could reduce oxidative stress in the striatum produced by 3-nitropropionic acid (3-NP), a mitochondrial toxin that irreversibly inhibits succinate dehydrogenase (SDH), causing impairment of energy metabolism, oxidative stress and a selective degeneration of striatal cells.

Methods: Rats were subjected to WBH (42°C) or normothermia control conditions for 30 min and then treated with 3-NP. Striatum samples were processed and the levels of protein carbonyl groups, biogenic amines, Hsp72 and salicylate hydroxylation (to probe the hydroxyl radical (OH•) intervention) were determined.

Results: WBH significantly reduced oxidative stress in the striatum of animals treated with 3-NP, as judged by reductions in protein carbonyl and salicylate hydroxylation derivative levels, whereas striatal Hsp72 expression was significantly increased. The groups treated with 3-NP presented an increased in the dopamine (DA) derivatives 2,3-dihydroxyphenylacetic acid (DOPAC) and norepinephrine (NE) concentration, whereas the striatal relation DOPAC/DA concentration indicate a reduced dopamine turnover.

Conclusions: These studies show, for the first time, that a heat shock pretreatment can ameliorate the oxidative stress produced by a metabolic toxin (3-NP) capable of impairing energy supply and produce selective striatal degeneration. These data contribute to a better understanding of the potential for thermal stress to modulate the type of oxidative stress usually present in neurodegenerative disorders associated with metabolic defects.

This should get you started, good luck. Now about your brain...

[HAL 9000 96]

Look for GroEL (Hsp60) + cell distribution. I think that physiologically speaking, whatever Hsp60 could be doing cytosolically can be upregulated using other pathways that might not need so much manipulation from a clinical perspective.

I will, but you know that I won't be sleeping tonight thinking about this. In my mind I keep imagining some brute force form of Le Chatelier's principle and just pumping so many healthy hsp60 units in there that there might be some turnover and the active units would somehow replace the defective units. Just one of the many things that I thought of.

Just admit it. What you want is for me to ship... lets say... some attenuated Adenovirus strain with... say... a CMV-driven Hsp60 cassette... for you to inject into people. Sick I tell you!!!

Was I that obvious? I mean perish the thought I would never do such a thing. Psst, how soon can you ship?

Maybe one day I get to work in that field. Converting protein into something of use with computers. In real life, if you meet an Asian (who is a Cambodian) guy at any physiology conference who is from Massachusetts, mention VJ. I like to work with cellular structures someday. Not with medicinal (since I'm not an expert) purposes, but for computer research purposes.

How many times have I heard that excuse.

Dude... I have the Dry ice box 5 feet away from the minus 80 freezer...

Send me your FedEx O/N account# LOL

* Disclaimer:

HAL 9000 does not deal with weaponizable viral agents.

Hi guys. I see you've been having a very good scientists discussion over here!

I am going to tell you the same thing "I am not an expert" in this topic... so I can't contribute to science now ha ha!

Thank you all for helping me, it should be great if you can request the article for me. I agree, it's a shame that only certain "hosters" (I don't know if it's the right name) or databases carry this kind of articles...

man, why getting into science has to be hard? science has to be for free!

Lots more journals are allowing NIH to index them and include free content after a while on PubMed.gov

I wish more would just allow open access like PLoS.

Then again, old boys prestige clubs die very, very hard.

[IR5FORMUMSIE 850]

Look for GroEL (Hsp60) + cell distribution. I think that physiologically speaking, whatever Hsp60 could be doing cytosolically can be upregulated using other pathways that might not need so much manipulation from a clinical perspective.

I will, but you know that I won't be sleeping tonight thinking about this. In my mind I keep imagining some brute force form of Le Chatelier's principle and just pumping so many healthy hsp60 units in there that there might be some turnover and the active units would somehow replace the defective units. Just one of the many things that I thought of.

Just admit it. What you want is for me to ship... lets say... some attenuated Adenovirus strain with... say... a CMV-driven Hsp60 cassette... for you to inject into people. Sick I tell you!!!

Was I that obvious? I mean perish the thought I would never do such a thing. Psst, how soon can you ship?

Dude... I have the Dry ice box 5 feet away from the minus 80 freezer...

Send me your FedEx O/N account# LOL

* Disclaimer:

HAL 9000 does not deal with weaponizable viral agents.

Just frozen bananas?

[NickD 202]

I recently learned that I can go to the IRS site and download any of their 1,025 tax forms, FOR FREE! That doesn't include all of the publications that you can also download FOR FREE!

[w¡n9Nµ7 §£@¥€r 123]

I recently learned that I can go to the IRS site and download any of their 1,025 tax forms, FOR FREE! That doesn't include all of the publications that you can also download FOR FREE!

[Niels Bohr 72]

I got the article!

PM me!

[Niels Bohr 72]

Try downloading through this link. Once you get it, PM me immediately. I need to take it down before I get in trouble.